We have compared PTC3 in our laboratories to another popular contamination control product, and found that it performs equivalently in reducing bacterial and fungal growth. Shown below in Figure 1, both Bacillus subtilis, Escherichia coli, and Agrobacterium tumefaciens inoculated in log-phase growth to 1X Murashige & Skoog (MS) liquid with 0.2% (v/v) PTC3 or 0.2% (v/v) competitor product did not grow to the extent that it did in just 1X Murashige & Skoog liquid media over 16 hours at 25°C and shaking at 125 RPM.
Figure 1. Bacterial Growth Inhibition of PTC3 (0.2 v/v%) and a similar product (0.2 v/v%) in 1X liquid MS.
PTC3 was also shown to inhibit fungal growth of Aspergillus niger on 1X Murashige & Skoog gelled media. Through 7 days at 25°C, no fungal growth was observed on cultures containing 0.2% (v/v) PTC3 or 0.2% (v/v).
The components of this product can be bound by proteins in media (e.g. peptone, casein) and rendered inactive.
At PhytoTechnology Laboratories we believe that there is no substitute for proper explant disinfection in the maintenance of long-term stock cultures. However for educational demonstration purposes or hobbyist’s looking to try plant tissue culture, PTC3 will allow you to perform routine tissue culture less stringently with a lower concern of losing your tissue culture to everyday contamination.
Please Note: While PhytoTechnology Laboratories® tests each lot of this product with two or more plant cell/ tissue culture lines, it is the sole responsibility of the purchaser to determine the appropriateness of this product for the specific plants that are being cultured and applications that are being used.